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KMID : 0545119950050060315
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 6 p.315 ~ p.318
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Transport of Sulfanilic Acid via Microbial Dipeptide Transport System
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Hwang, Se Young
Ki, Mi Ran/Cho, Suk Young/Yoo, Ick Dong
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Abstract
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Sulfanilic acid (4-aminobenzenesulfonic acid) alone is normally not permeant in bacteria but can be readily delivered via the microbial dipeptide transport system. A dipeptidyl derivative of this compound, L-phenylalanyl-L-2-sulfanilylglycine (PSG), prepared by attachment of its primary amino group to the phenylalanyl α-glycine moiety, appeared to have a Km of 0.125 mM and a Vmax of 1.9 nmoles/㎖/min (A_660, 1.0) in Escherichia coli. From competitive spectrophotometric analysis, it was found that the type of amino acids in both of the N-and C-terminals affected the kinetic power of dipeptides. The growth inhibitory effect of PSG was over 7 times more potent than that of the sulfanilic acid against E. coli, suggesting that this potential inhibition was presumably due to the increased hydrophobic nature of the sulfanilyl dipeptide.
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